PSILOCYBIN QTest® The First Psilocybin Potency Test Kit for Magic Mushrooms
Have you ever wondered, "How strong are my magic mushrooms?" Magic mushroom potency can be wildly unpredictable, varying between strains, or even from one mushroom to another in the same batch.
That’s why we created the PSILOCYBIN QTest, the world’s first scientifically validated psilocybin test kit designed to measure the exact amount of psilocybin in your mushrooms. Whether you're new to the scene or an experienced user, our PSILOCYBIN QTest provides an easy, reliable, and accurate way to test your mushrooms from the comfort of your home.
Why Measure Mushroom Potency?
In a world where psychedelics are increasingly recognized for their therapeutic benefits and much more, having access to reliable, scientific data on mushroom potency is more important than ever. At miraculix, we’re proud to offer a solution that empowers users to take control of their psychedelic experiences in a safer, informed way. The PSILOCYBIN QTest provides the accuracy and confidence needed for a safer psychedelic journey.
Developed for Home-use
- Rapidly know how strong your mushrooms are
- Minimal sample required, user-friendly testing
- Grind up a whole batch and just test one sample
- University validated method
- The only mushroom potency test on the market
PSILOCYBIN QTest® Color Series
The color series shows the individual color levels for the evaluation of the PSILOCYBIN QTest. Each color thus stands for a direct color field. This would mean that we see the following values from left to right:
- 0% - 0.3% - 0.6% - 0.9% - 1.2% - 1.5% - 1.8% - 2.1% - 2.4%
This illustration is intended to help you with the evaluation if you have difficulties in estimating the correct color field. Please remember that colors can look different on every screen. The images are therefore only intended as a support and assessment.
What is inside the PSILOCYBIN QTest® Kit
- Box with label
- Evaluation scale with quick-reference guide
- Detailed instructions in various languages
- Glass vessel with detection solution
- Plastic vessel with extraction solution
- 1 ml syringe
- Blunt needle
- Sterifilt (filter for extraction)
There is also a PRO VERSION
Whether you're an experienced user, a grower, or a scientist, our affordable QTest-Photometer is designed to meet your needs. It is a precalibrated photometer which display you a precise amount of psilocybin, and works with other QTests as well.
Wanna test larger quantities? We offer solutions for that too! In combination with our spectrophotometer, we’ve developed the Psilocybin BulkQTest, designed specifically for use with the QTest photometer. This kit is perfect for high-precision analysis while keeping costs low.
US VERSUS THEM
What distinguishes our psilocybin QTest from qualitative reagents such as Ehrlich reagent when testing magic mushrooms:
- Quantiative Results in 30min
- Precise detection of Psilocybin and Psilocin
- Reliable across all natural sample types
- Accurate at various concentrations
Analyze Mushroom Potency in 3 easy steps
Preparation & Extraction
Simply chop and homogenize your dried mushroom material (0.15 g) and add it to the solvent reagent for extraction.
Detection
Add 1 ml from the extraction solution to the detection vial and incubate in hot water.
Evaluation
Compare the color intensity with the color chart and determine the psilocybin potency of your mushrooms.
Step-by-step Guide for Determining Magic Mushrooms
In this video, we show you all the components that you will receive when you are supplied with a Psilocybin QTest. We also show you all the steps involved in carrying out the quick test. We have used different mushrooms containing psilocybin to show the differences and fluctuations in the active ingredient content. This can be seen particularly well in the evaluation step.
The Science Behind: Validated and Proven Accuracy
- Scientifically Validated: The PSILOCYBIN QTest is the first scientifically validated psilocybin potency test available for home use.
- Lab-Grade Accuracy: Our test has been rigorously compared to professional lab methods like HPLC (High-Performance Liquid Chromatography), providing you with results you can trust.
- Trusted by Experts: Leading mycologists and researchers, like Alan Rockefeller, rely on the PSILOCYBIN QTest for testing rare mushroom species and conducting in-depth studies.
Our PSILOCYBIN QTest has undergone thorough validation to ensure accuracy and reliability. The test follows the ICH Q2(R1) pharmaceutical validation guidelines, which are typically used to ensure the safety and precision of active ingredients in human medicine. This means you can trust the potency measurements provided by our test kit. To see the full validation data and comparison curve, click here to view the validation sheets.
Empower Safer Choices
Are You Convinced?
buy A QTEST NOW AND FINANCE NEW RESEARCH
Order your PSILOCYBIN QTest now and directly contribute to new research and development of new QTest kits.
With every purchase in our webshop, you’re supporting a small independent university start up and advancing harm reduction efforts and psychedelic research. Our mission is to empower everyone to make informed, self-determined decisions with trustworthy, lab-grade test kits.
A tool for Science & More
The PSILOCYBIN QTest is more than just a mushroom potency test kit—it's a tool that ia being used for a community research project. In collaboration with Charité – Universitätsmedizin Berlin, this project allows users to anonymously submit their results and experiences, contributing to the progress of psychedelic science. Your data helps us explore how psilocybin content varies across different species and strains, with the aim of publishing the findings as open-source for the scientific community.
Knowledge is power! By joining this mission and collaborating together, we can finally answer many open questions and connect the missing pieces of evidence.
Frequently asked questions about the PSILOCYBIN QTest®
The PSILOCYBIN QTest is the first scientifically validated home-use test kit designed to measure the psilocybin potency of magic mushrooms. It provides accurate, reliable data on the psilocybin and psilocin content in your mushrooms in just 30 minutes.
Absolutely! The PSILOCYBIN QTest is perfect for testing the potency of any psilocybin-containing mushrooms, including popular species like Psilocybe cubensis, truffles, and even mycelium. It requires only 0.15 g of dried material to perform the test.
No, the PSILOCYBIN QTest is specifically designed for use with dried fungal materials, such as mycelium, fruiting bodies, or truffles. Fresh mushrooms contain a high and fluctuating water content, which can interfere with accurate measurements in this psilocybin test.
Yes! In addition to the application on dried fungal material, the rapid test shows equally reliable results for most solvent-free extracts.
Extracts are usually more concentrated. To ensure that the color result of the PSILICYBIN QTEST still fits the scale on the evaluation card, you have to reduce the amount of starting material accordingly. Before testing homogenize your substance. Typically, methanol extracts are triple-concentrated, so if you have a crude extract, we recommend dividing the amount you use for the test by the factor three (50 mg). If the extract is considerably more concentrated, reduce the initial quantity accordingly. The extract must be as solvent-free as possible, as this can interfere with the extraction solution.
Remember that you have to multiply the result of the color scale in the end with the used factor to get the actual result for the remaining sample. For example, weigh 50 mg (0.05 g) of material for a 3X extract. Weigh 15 mg (0.015 g) of material for a 10X extract.
No, the PSILOCYBIN QTest is specifically designed to measure psilocybin and psilocin content in magic mushrooms and does not work with synthetic substances like 4-AcO-DMT.
The PSILOCYBIN QTest quantifies the total alkaloid content (psilocybin + psilocin) and gives the added values in the form of a total concentration of psilocybin.
The background for this is that only the very stable active ingredient psilocybin is naturally present in healthy fungal tissue. For example, if not properly dried and stored, degradation of the prodrug psilocybin to unstable psilocin, the actual pharmacological active ingredient, occurs. Even if this occurs, the PSILOCYBIN QTest will give you information about the total amount of both alkaloids. Both substances have different molecular weights and to avoid a complicated conversion we give the value in terms of the well-known alkaloid psilocybin.
The PSILOCYBIN-QTEST works well with, for example, rotated or evaporated ("dry") extracts, but also some solvents like ethanol work if the concentration of alkaloids is not too low. This means that if too many foreign solvents are added to our extraction, it will disturb our extraction system.
The best way would be to evaporate the ethanol or any other solvent from a defined amount, note the dry weight, and measure it with the test.
The PSILOCYBIN QTest has a shelf life of at least 18 months if stored properly. Keep the reagents in the refrigerator at (35 - 46 °F/ 2 - 8 °C) and out of direct sunlight. Over time, the extraction liquid may turn slightly yellow, that’s okay. It is still usable.
Yes we have a validation for our PSILOCYBIN-QTests. This is a voluntarily validated and standardized test procedure based on the pharmaceutical method validation guideline ICH Q2(R1), which we have extended to the QTests. This guideline describes the requirements of the analytical method for active ingredients that are subsequently to be used in human medicine and guarantees a safe dosage of these active ingredients, for example in pharmacies.
You can find the short report Validation here: Validation report
Yes and Yes! We would very much like to offer access to our instructions at any time. That's why we offer the possibility to view the instructions digitally and to use them for the execution. Furthermore, we would like to expand the global access and make other languages available. For this we are busy translating the individual instructions. The current instructions for our test kit can be found here:
EN (English): PSILOCYBIN-QTest-Manual-English
D (German): PSILOCYBIN-QTest-Manual-German
NL (Dutch:) PSILOCYBIN-QTest-gids-Nederlands
We recommend ensuring that floating fungi are not sucked into the system and positioning the sterile filter below the floating fungus layer before applying vacuum. If the process fails initially, cleaning the filter very carefully and reapplying the syringe can help. The test can still function if fungal components are transferred, but this may complicate color evaluation. Link to the video.
No, the test is single-use. However, we offer multi-test packs if you need to test several batches or strains.
You still have open questions?
We could not answer your questions completely on this page? Then write us your questions about our QTest quickly and easily in the form.
About the author
Dr. Felix Blei – Scientific Author
Dr. Felix Blei is the CEO and Founder of miraculix Lab, and a Postdoctoral Researcher at the Friedrich Schiller University Jena. With a PhD (Dr. rer. nat.) in Microbiology and a strong background in natural product biosynthesis, he is internationally recognized for his pioneering work on psychoactive fungi—particularly the biosynthesis of psilocybin and related compounds.
During his doctoral research at the Institute of Pharmaceutical Microbiology under Prof. Dirk Hoffmeister, Dr. Blei was the first to elucidate the full biosynthetic pathway of psilocybin in Psilocybe mushrooms. He further developed an in vitro system capable of producing psilocybin, serotonin, and novel non-natural analogues. His discovery of naturally occurring β-carbolines in Psilocybe—compounds that may synergistically enhance psilocybin's neurotropic effects—led to the concept of “psilohuasca,” a naturally occurring combination of MAO inhibitors and psychedelics in fungi.
Building on his academic expertise, Dr. Blei developed the first reliable rapid tests for psilocybin, which laid the foundation for the spin-off company miraculix. Today, miraculix provides quantitative drug checking tools that are used throughout Germany and Europe. He also leads the German ALIVE project—an evidence-based drug checking initiative funded by state governments, offering mobile substance analysis and harm reduction at music festivals and public events.
With over 500 citations, numerous publications in high-impact journals, and a passion for accessible science, Dr. Blei bridges cutting-edge research with real-world application. His work contributes to safer substance use, public health strategies, and a deeper understanding of natural psychoactive compounds.
You can learn more about him on LinkedIn or read his publications on Researchgate
