Psilocybin QTest® The First Psilocybin Test Kit for Psilocybin Potency
Have you ever wondered, "How strong are my magic mushrooms?" Magic mushroom potency can be wildly unpredictable, varying between strains, or even from one mushroom to another in the same batch.
That’s why we created the Psilocybin QTest, the world’s first scientifically validated psilocybin test kit designed to measure the exact amount of psilocybin in your mushrooms. Whether you're new to the scene or an experienced user, our Psilocybin QTest provides an easy, reliable, and accurate way to test your mushrooms from the comfort of your home.
Why use an Psilocybin Test Kit?
In a world where psychedelics are increasingly recognized for their therapeutic benefits and much more, having access to reliable, scientific data on mushroom potency is more important than ever. At miraculix, we’re proud to offer a solution that empowers users to take control of their psychedelic experiences in a safer, informed way. The Psilocybin QTest kit provides the accuracy and confidence needed for a safer psychedelic journey.
What the Psilocybin Test Kit Can Do
- Rapidly know how strong your mushrooms are
- Minimal sample required, user-friendly testing
- Grind up a whole batch and just test one sample
- University validated method
- The only mushroom potency test on the market
Easy 3-Step Process
The Psilocybin Test Kit provides a quick and simple way to measure psilocybin potency in just three steps. Whether at home or in a lab, the Psilocybin QTest delivers accurate results in minutes.
- Step 1: Preparation
Place a small sample (0.15 grams) of dried mushrooms into the extraction vial. - Step 2: Extraction
Add extraction solution to isolate the psilocybin for precise measurement. - Step 3: Reaction & Results
Compare the color change to the reference chart, which ranges from 0% to 2.4% potency.
This simple process ensures accurate, reliable results every time with the Psilocybin Test Kit.
Easy-to-Read Results
The Psilocybin Test Kit offers quick visual feedback with color changes in the vials, allowing you to easily identify the psilocybin potency of your sample. Whether it's no psilocybin, a light concentration, or a high potency, the included reference chart ensures accurate measurement, from 0% to 2.4% psilocybin.
Immediate Feedback: Instantly determine if your sample has no psilocybin, low potency, or strong psilocybin concentration.
Clear Color Indicators: The vials show distinct color changes, ranging from clear to deep red, indicating varying psilocybin levels.
Precise Measurement: Use the reference chart for an accurate and reliable reading of psilocybin potency.
How the Psilocybin Test Kit Measures Mushroom Potency
The Psilocybin Test Kit works via a colorimetric scale, where each color corresponds to a specific psilocybin potency level. This makes it easy to assess the potency of your mushroom sample without the need for complex equipment. Simply compare the sample’s color reaction to the reference chart, and you’ll get a clear visual estimate of the psilocybin concentration, from 0 % to 2.4 %.
| Psilocybin Potency (%) | Color Description |
| 0 % (0 mg) | clear, slight yellow tone |
| 0,6 % (6 mg) | light brown |
| 1,2 % (12 mg) | brown |
| 1,8 % (18 mg) | dark brown |
| 2,4 % (24 mg) | almost black |
What is inside the Psilocybin Test Kit
- Box with Psilocybin QTest label
- Evaluation scale with quick-reference guide
- Detailed instructions in various languages
- Glass vessel with detection solution
- Plastic vessel with extraction solution
- 1 ml syringe
- Blunt needle
- Sterifilt (filter for extraction)
There is also a PRO VERSION
Whether you're an experienced user, a grower, or a scientist, our affordable QTest-Photometer is designed to meet your needs. It is a precalibrated photometer which display you a precise amount of psilocybin, and works with other QTests as well.
Wanna test larger quantities? We offer solutions for that too! In combination with our spectrophotometer, we’ve developed the Psilocybin BulkQTest, designed specifically for use with the QTest photometer. This kit is perfect for high-precision analysis while keeping costs low.
US VERSUS THEM
What distinguishes our Psilocybin potency test from qualitative psilocybin test kits such as Ehrlich reagent when testing magic mushrooms:
- Quantiative Results in 30min
- Precise detection of Psilocybin and Psilocin
- Reliable across all natural sample types
- Accurate at various concentrations
Test Psilocybin Mushroom Potency in 3 easy steps
Preparation and Extraction
To begin testing your psilocybin sample, start by finely chopping and homogenizing approximately 0.15 grams of your dried mushroom material. Once the material is evenly ground, add it to the solvent reagent included in your psilocybin test kit for extraction. This step ensures that the psilocybin compounds are properly dissolved and ready for accurate testing, allowing you to measure the potency of your mushrooms with precision.
Incubation and Detection
After preparing your sample, add 1 ml of the extraction solution to the detection vial provided in your psilocybin potency test kit. Next, incubate the vial in hot water to ensure optimal conditions for the detection reaction. This crucial step helps to activate the reagents, allowing for an accurate measurement of psilocybin potency in your sample.
Evaluation of Potency
Compare the Color Intensity: Once the reaction is complete, compare the color intensity in your detection vial with the provided color chart. This will help you accurately determine the psilocybin potency of your mushrooms using the psilocybin test kit. The more intense the color change, the higher the concentration of psilocybin in your sample, allowing for a precise and reliable assessment of its potency.
Step-by-step Video for Determining Magic Mushrooms
In this video, we show you all the components that you will receive when you are supplied with a Psilocybin QTest - the first psilocybin potency test. We also show you all the steps involved in carrying out the quick test. We have used different mushrooms containing psilocybin to show the differences and fluctuations in the active ingredient content. This can be seen particularly well in the evaluation step.
The Science Behind: Validated and Proven Accuracy
- Scientifically Validated: The Psilocybin QTest is the first scientifically validated psilocybin potency test kit available for home use.
- Lab-Grade Accuracy: Our test has been rigorously compared to professional lab methods like HPLC (High-Performance Liquid Chromatography), providing you with results you can trust.
- Trusted by Experts: Leading mycologists and researchers, like Alan Rockefeller, rely on the PSILOCYBIN QTest for testing rare mushroom species and conducting in-depth studies.
Our Psilocybin potency test has undergone thorough validation to ensure accuracy and reliability. The test follows the ICH Q2(R1) pharmaceutical validation guidelines, which are typically used to ensure the safety and precision of active ingredients in human medicine. This means you can trust the potency measurements provided by our test kit. To see the full validation data and comparison curve, click here to view the validation sheets.
CASE Report
Psilocybin Test Kit Validation and Case Report
In this Case Report, we demonstrate the precision and reliability of the Psilocybin Test Kit for measuring psilocybin potency. Dried Psilocybe cubensis Albino AA+ fruiting bodies were first finely ground. Different amounts of this powdered biomass were accurately weighed and placed into separate Eppendorf tubes for extraction.
Each sample was individually extracted and filtered, then incubated with the test kit's detection solution in cuvettes. After incubation, the samples were measured using the QTest Photometer at 595 nm, providing accurate results based on the color change.
Simultaneously, three 50 mg samples were extracted and analyzed using HPLC to determine the exact psilocybin content, ensuring the accuracy of the results.
The resulting plot shows a highly linear correlation between the color change and psilocybin concentration, confirming the effectiveness of the Psilocybin QTest. The data exhibits a strong linear relationship (R² = 0.9984), validating the Psilocybin Test Kit as a reliable method for measuring psilocybin content.
This experiment highlights the precision and reliability of the Psilocybin Potency Test, demonstrating how the Psilocybin QTest provides an easy-to-use, scientifically backed solution for determining mushroom potency with confidence.
Homogenization of dried fruiting bodies from Psilocybe cubensis "AA+"
Ascending amounts of the sample in each Eppendorf tube
Perfect color series after incubation with detection solution
Plot shows nearly perfect linearity of the Psilocybin Test kit
Empower Safer Choices
Are You Convinced?
buy A Psilocybin test kit NOW AND FINANCE NEW RESEARCH
Order your Psilocybin test kit now and directly contribute to new research and development of new QTest kits.
With every purchase in our webshop, you’re supporting a small independent university start up and advancing harm reduction efforts and psychedelic research. Our mission is to empower everyone to make informed, self-determined decisions with trustworthy, lab-grade test kits.
A tool for Science & More
The Psilocybin test kit is more than just a psilocybin potency test—it's a tool that is being used for a community research project. In collaboration with Charité – Universitätsmedizin Berlin, this project allows users to anonymously submit their results and experiences, contributing to the progress of psychedelic science. Your data helps us explore how psilocybin content varies across different species and strains, with the aim of publishing the findings as open-source for the scientific community.
Knowledge is power! By joining this mission and collaborating together, we can finally answer many open questions and connect the missing pieces of evidence.
Behind Miraculix: Dr. Felix Blei and the Development of the Psilocybin Potency Test Kit
Dr. Felix Blei, CEO and founder of Miraculix, began his career researching psilocybin and other psychoactive fungi, earning his PhD in microbiology. His groundbreaking work on the biosynthesis of psilocybin in Psilocybe mushrooms laid the foundation for the Psilocybin Test Kit named Psilocybin QTest. As the first to fully elucidate the psilocybin biosynthetic pathway, Dr. Blei's discoveries paved the way for the development of quantitative psilocybin testing.
In 2020, Dr. Blei founded Miraculix to apply his research to practical solutions. The Psilocybin QTest was created as the first reliable test for psilocybin potency, empowering users to safely measure the strength of their mushrooms. With his extensive background in natural product biosynthesis, Dr. Blei’s work continues to contribute to harm reduction and safer psychedelic use worldwide.
Through Miraculix, Dr. Blei bridges cutting-edge research with real-world applications, providing tools for both researchers and users to better understand psilocybin and its effects.
Psilocybin Blogs
Explore More with the Psilocybin Test Kit Blog
The psilocybin test kit is more than just a tool — it’s your key to understanding mushroom potency, storage safety, and responsible use. In our latest blog highlights, you’ll learn how to spot spoiled mushrooms, how long psilocybin lasts, and whether it can be detected in a drug screening. Whether you’re using the mushroom test kit to analyze homegrown shrooms or check aged samples for potency loss, these expert guides provide the insights you need to stay informed and safe.
Do Magic Mushrooms Go Bad? Learn How to Spot Spoilage & Test Potency
Not sure if your old stash is still safe or strong? Discover how psilocybin degrades, what blue bruising really means, and how to detect spoiled mushrooms before it’s too late. This guide breaks down storage tips, mold warnings, and how the psilocybin test kit helps you check for potency loss at home.
Magic Mushroom Knowledge Hub: Cultivation, Chemistry & Psilocybin Testing
From growing your own mushrooms to understanding how psilocybin works—our Magic Mushroom Hub covers it all. Learn how to safely use the psilocybin test kit, understand brain effects, legal status, microdosing, and how to identify different strains.
Psilocybin Drug Tests: Can You Be Detected? Learn the Facts
Worried about drug tests after a mushroom trip? Learn what types of drug screenings can detect psilocybin or psilocin—and why most standard tests won’t. This article explains detection windows, metabolic breakdown, and how it differs from the psilocybin test kit.
Frequently asked questions about the miraculix
psilocybin mushroom test kit
Got questions about the QTest® for magic mushroom potency? We’ve got answers.
If you are looking for a reliable mushroom test kit, this FAQ covers everything you need to know about the Psilocybin QTest®.
From how it works to what it detects (and doesn’t), find all essential details below.
The Psilocybin QTest is the first scientifically validated home-use psilocybin test kit designed to measure the psilocybin potency of magic mushrooms. It provides accurate, reliable data on the psilocybin and psilocin content in your mushrooms in just 30 minutes.
Absolutely! The PSILOCYBIN QTest is perfect for testing the potency of any psilocybin-containing mushrooms, including popular species like Psilocybe cubensis, truffles, and even mycelium. It requires only 0.15 g of dried material to perform the test.
No, the PSILOCYBIN QTest is specifically designed for use with dried fungal materials, such as mycelium, fruiting bodies, or truffles. Fresh mushrooms contain a high and fluctuating water content, which can interfere with accurate measurements in this psilocybin test.
Yes! In addition to the application on dried fungal material, the rapid test shows equally reliable results for most solvent-free extracts.
Extracts are usually more concentrated. To ensure that the color result of the PSILICYBIN QTEST still fits the scale on the evaluation card, you have to reduce the amount of starting material accordingly. Before testing homogenize your substance. Typically, methanol extracts are triple-concentrated, so if you have a crude extract, we recommend dividing the amount you use for the test by the factor three (50 mg). If the extract is considerably more concentrated, reduce the initial quantity accordingly. The extract must be as solvent-free as possible, as this can interfere with the extraction solution.
Remember that you have to multiply the result of the color scale in the end with the used factor to get the actual result for the remaining sample. For example, weigh 50 mg (0.05 g) of material for a 3X extract. Weigh 15 mg (0.015 g) of material for a 10X extract.
No, the PSILOCYBIN QTest is specifically designed to measure psilocybin and psilocin content in magic mushrooms and does not work with synthetic substances like 4-AcO-DMT.
The PSILOCYBIN QTest quantifies the total alkaloid content (psilocybin + psilocin) and gives the added values in the form of a total concentration of psilocybin.
The background for this is that only the very stable active ingredient psilocybin is naturally present in healthy fungal tissue. For example, if not properly dried and stored, degradation of the prodrug psilocybin to unstable psilocin, the actual pharmacological active ingredient, occurs. Even if this occurs, the PSILOCYBIN QTest will give you information about the total amount of both alkaloids. Both substances have different molecular weights and to avoid a complicated conversion we give the value in terms of the well-known alkaloid psilocybin.
The PSILOCYBIN-QTEST works well with, for example, rotated or evaporated ("dry") extracts, but also some solvents like ethanol work if the concentration of alkaloids is not too low. This means that if too many foreign solvents are added to our extraction, it will disturb our extraction system.
The best way would be to evaporate the ethanol or any other solvent from a defined amount, note the dry weight, and measure it with the test.
The PSILOCYBIN QTest has a shelf life of at least 18 months if stored properly. Keep the reagents in the refrigerator at (35 - 46 °F/ 2 - 8 °C) and out of direct sunlight. Over time, the extraction liquid may turn slightly yellow, that’s okay. It is still usable.
Yes we have a validation for our PSILOCYBIN-QTests. This is a voluntarily validated and standardized test procedure based on the pharmaceutical method validation guideline ICH Q2(R1), which we have extended to the QTests. This guideline describes the requirements of the analytical method for active ingredients that are subsequently to be used in human medicine and guarantees a safe dosage of these active ingredients, for example in pharmacies.
You can find the short report Validation here: Validation report
Yes and Yes! We would very much like to offer access to our instructions at any time. That's why we offer the possibility to view the instructions digitally and to use them for the execution. Furthermore, we would like to expand the global access and make other languages available. For this we are busy translating the individual instructions. The current instructions for our test kit can be found here:
EN (English): PSILOCYBIN-QTest-Manual-English
D (German): PSILOCYBIN-QTest-Manual-German
NL (Dutch:) PSILOCYBIN-QTest-gids-Nederlands
We recommend ensuring that floating fungi are not sucked into the system and positioning the sterile filter below the floating fungus layer before applying vacuum. If the process fails initially, cleaning the filter very carefully and reapplying the syringe can help. The test can still function if fungal components are transferred, but this may complicate color evaluation. Link to the video.
No, the test is single-use. However, we offer multi-test packs if you need to test several batches or strains.
You still have open questions about our Psilocybin test kit?
We could not answer your questions completely on this page? Then write us your questions about our psilocybin test kit quickly and easily in the form.
About the author
Dr. Felix Blei – Scientist and Scientific Author
Dr. Felix Blei is the CEO and Founder of miraculix Lab, and a Postdoctoral Researcher at the Friedrich Schiller University Jena. With a PhD (Dr. rer. nat.) in Microbiology and a strong background in natural product biosynthesis, he is internationally recognized for his pioneering work on psychoactive fungi—particularly the biosynthesis of psilocybin and related compounds (Blei, F., 2020).
During his doctoral research at the Institute of Pharmaceutical Microbiology under Prof. Dirk Hoffmeister, Dr. Blei was the first to elucidate the full biosynthetic pathway of psilocybin in Psilocybe mushrooms (Fricke, Blei et al., 2017) Angewandte Chemie. He further developed an in vitro system capable of producing psilocybin, serotonin, and novel non-natural analogues (Blei et al., 2018) Chemistry- A European Journal . His discovery of naturally occurring β-carbolines in Psilocybe—compounds that may synergistically enhance psilocybin's neurotropic effects—led to the concept of “psilohuasca,” a naturally occurring combination of MAO inhibitors and psychedelics in fungi (Blei, Dörner et al. 2020) Chemistry–A European Journal.
Building on his academic expertise, Dr. Blei developed the first reliable rapid tests for psilocybin, which laid the foundation for the spin-off company miraculix. Today, miraculix provides quantitative drug checking tools that are used throughout Germany and Europe. He also leads the German ALIVE project—an evidence-based drug checking Initiative funded by state governments, offering mobile substance analysis and harm reduction at music festivals and public events.
With over 500 citations, numerous publications in high-impact journals, and a passion for accessible science, Dr. Blei bridges cutting-edge research with real-world application. His work contributes to safer substance use, public health strategies, and a deeper understanding of natural psychoactive compounds.
You can learn more about him on LinkedIn or read his publications on Researchgate
